2019-02-19 18:23:412018-02-23 23:00:09
geneLength
633
630
Gene
Coordinates
3,949,952 3,950,584
3,949,952 → 3,950,584
Gene
Phenotypes of a mutant
a ''[[gene|relA]] [[gene|sasA]] [[gene|sasB]]'' triple mutant requires branched chain amino acids, methionine and threonine for growth, the requirement can be suppressed by reduced expression of ''[[gene|guaA]]'' or inactivation of ''[[gene|codY]]'' [Pubmed|24163341]
a ''[[gene|relA]] [[gene|sasA]] [[gene|sasB]]'' triple mutant acquires suppressor mutations in ''[[gene|guaA]], [[gene|guaA]], [[gene|gmk]]'' or inactivation of ''[[gene|codY]]'' [Pubmed|24682323,24163341]
a ''[[gene|relA]] [[gene|sasA]] [[gene|sasB]]'' triple mutant requires branched chain amino acids, methionine and threonine for growth, the requirement can be suppressed by reduced expression of ''[[gene|guaB]]'' or inactivation of ''[[gene|codY]]'' [Pubmed|24163341]
a ''[[gene|relA]] [[gene|sasA]] [[gene|sasB]]'' triple mutant acquires suppressor mutations in ''[[gene|guaA]], [[gene|guaB]], [[gene|gmk]]'' or inactivation of ''[[gene|codY]]'' [Pubmed|24682323,24163341]
The protein
Structure
[PDB|6FGK] [Pubmed|29391580]
[PDB|5DEC] ([[protein|SasB]], 39% identity, 70% similarity) [Pubmed|26460002]
Biological materials
Mutant
MGNA-B218 (ywaC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1217 NBRP B. subtilis, Japan]
GP2066 (''[[gene|sasA]] [[gene|sasB]] [[gene|relA]]::mls''), available in [SW|Jrg Stlke]'s lab
BKE38480 ([[gene|sasA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTCGTCATCTCCTTTA, downstream forward: _UP4_TAAAAAAGACGGCACCCAAG
BKK38480 ([[gene|sasA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTCGTCATCTCCTTTA, downstream forward: _UP4_TAAAAAAGACGGCACCCAAG
MGNA-B218 (ywaC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1217 NBRP B. subtilis, Japan]
GP2066 (''[[gene|sasA]] [[gene|sasB]] [[gene|relA]]::mls''), available in [SW|Jörg Stülke]'s lab
BKE38480 (Δ[[gene|sasA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTCGTCATCTCCTTTA, downstream forward: _UP4_TAAAAAAGACGGCACCCAAG
BKK38480 (Δ[[gene|sasA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTCGTCATCTCCTTTA, downstream forward: _UP4_TAAAAAAGACGGCACCCAAG
References
Original publications
ec